Responsibilities:
- Develop, optimize, and validate molecular biology methods for gene and cell therapy applications (e.g., VCN, gene editing proficiency, genomic titer, RCL, residual DNA).
- Perform molecular cloning, DNA plasmid purification, and in vitro transcription to generate assay controls.
- Design, execute, and analyze digital PCR (dPCR) and qPCR assays using QIAGEN QIAcuity systems.
- Support assay development through CRISPR-based screen optimization and molecular assay validation.
- Collaborate cross-functionally to support QC testing and contract research service projects.
Requirements:
- PhD (1+ year industry) or MSc (3+ years industry).
- Strong experience with digital PCR (dPCR – QIAGEN QIAcuity).
- Demonstrated expertise in molecular cloning.
- Proven experience developing and validating dPCR and qPCR assays.
- Familiarity with lentivirus or retrovirus systems and mammalian cell lines.
Job Type: Full-time
Pay: $80,000.00 - $100,000.00 per year
Work Location: In person